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Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ 51–59 Protein in Mice

Publication year: 
Author (s): 
Garulli, B [et al.]
Publication details: 
s.l., Biomed Research International, 2014
Publication in: 
BioMed Research International Volume 2014, Article ID 904038, 10 p.

Recombinant influenza viruses hold promise as vectors for vaccines to preventt transmission of mucosal pathogens. In this study, we generated a recombinant WSN/TatΔ51–59 virus in which Tat protein lacking residues 51 to 59 of the basic domain was inserted into the N-terminus of the hemagglutinin (HA) of A/WSN/33 virus. The TatΔ 51–59 insertion into the viral HA caused a 2-log reduction in viral titers in cell culture, compared with the parental A/WSN/33 virus, and severely affected virus replication in vivo. Nevertheless, Tat-specific antibodies and T cell responses were elicited upon a single intranasal immunization of BALB/c mice
with WSN/TatΔ 51–59 virus. Moreover, Tat-specific immune responses were also detected following vaccine administration via the vaginal route.These data provide further evidence that moderately large HIV antigens can be delivered by chimeric HA constructs and elicit specific immune responses, thus increasing the options for the potential use of recombinant influenza viruses, and their derivatives, for prophylactic and therapeutic vaccines.